Journal: Frontiers in pharmacology
Article Title: Inhibition of adenylyl cyclase 1 by ST034307 inhibits IP 3 -evoked changes in sino-atrial node beat rate.
doi: 10.3389/fphar.2022.951897
Figure Lengend Snippet: FIGURE 2 IP3R2 is expressed in close proximity with AC1 in guinea pig SAN myocytes. (A) Representative example of a fixed, isolated guinea pig SAN myocyte immunolabelled for (i) IP3R2 (magenta), (ii) AC1 (cyan), (iii) co-immunolabelled for IP3R2 (magenta) and AC1 (cyan). Example brightfield image shown in (iv). (B) Intensity plot to show staining intensity along the white line shown in (A). R-value calculated from n = 7 cells. (C–E) Intensity surface plots showing the distribution of staining of IP3R2 [magenta, (D)] and AC1 [cyan, (E)] for the regions of cell shown in (C). Scale bars representing 10 µm are indicated in (A) and 2 µm in (C). For the purposes of presentation only, red and green channels have been represented as magenta and cyan, respectively.
Article Snippet: Mouse anti-IP3R monoclonal primary antibody IP3R2 (sc-398434) was purchased commercially (Santa Cruz Biotechnology, Santa Cruz, CA, United States) and used at a dilution of 1:50.
Techniques: Isolation, Staining